BACKGROUND Malaria is a disease that affects many tropical and subtropical countries, including Brazil. The use of tests for malaria detection is one of the fundamental strategies recommended by the World Health Organization for the control and eradication of the disease. The lack of diagnostic tests leads to an increase in transmission and non-reporting cases. OBJECTIVES This work described an electrochemical immunosensor for detecting Plasmodium vivax lactate dehydrogenase antigen (Ag-PvLDH). METHODS The device has developed by immobilising egg yolk IgY antibodies (Ab-PvLDH) on a gold electrode surface using cysteamine as linker. The immunosensor fabrication was followed by differential pulse voltammetry, and contact angle measurements were performed to characterise the modified gold electrode surface. FINDINGS The results for Ag-PvLDH determination exhibit a linear response at 10-50 µg mL-1 concentration range, with a limit of detection of 455 ng mL-1. The excellent selectivity of the device was confirmed. MAIN CONCLUSIONS The developed immunosensor showed a good performance, therefore, it can be considered an alternative test to detect malaria caused by P. vivax.

RESUMEN La interacción entre plantas y patógenos es una relación muy dinámica y compleja, que conlleva un alto grado de especificidad y es esta última característica, la que desencadena respuestas tan importantes en la supervivencia de uno u otro. El patosistema formado por tomate (Solanum lycopersicum L.) y Fusarium oxysporum f. sp. lycopersici (Fol) ha sido objeto de múltiples estudios, debido a la importancia de la hortaliza, a nivel mundial y por el impacto económico y ecológico del hongo, responsable de la marchitez vascular, provocando pérdidas que llegan hasta el 100%. Una forma de encontrar alternativas para el manejo de cualquier patosistema es conocer los actores involucrados y los mecanismos que rigen la interacción, a través de avances tecnológicos y científicos, que muestren, claramente, cómo se desarrolla la interacción, a nivel genético. Esta revisión recoge la información de fuentes científicas con énfasis en el conocimiento del hongo, el cultivo del tomate y la defensa vegetal, aplicada a este patosistema, así como los mecanismos moleculares.

ABSTRACT The interaction between plants and pathogens is a very dynamic and complex relationship that also includes a high degree of specificity, and it is precisely this last characteristic which triggers such important responses in the survival of one or the other. The pathosystem formed by tomato (Solanum lycopersicum L.) and Fusarium oxysporum f. sp. lycopersici (Fol) has been the subject of multiple studies due to the importance of the vegetable worldwide and for the economic and ecological impact of the fungus responsible for the vascular wilt disease in tomato, causing losses that go up to 100%. One way to find alternatives for the management of any pathosystem is to know the actors involved and the mechanisms that govern the interaction through technological and scientific advances that clearly show how the interaction develops on a genetic level. This review collects the information from different scientific sources with focus on the knowledge of the fungus, tomato cultivation and plant defense applied to this pathosystem, as well as the molecular mechanisms.

RESUMO Neste trabalho são aplicados corantes extraídos de plantas da Floresta Amazônica como sensibilizadores em células solares sensibilizadas por corantes. As plantas selecionadas foram Euterpe oleracea, Arrabidaea chica, Bixa orellana, Genipa americana e Myrcia sylvatica e os corantes foram coletados de frutos, folhas, sementes, polpa e sementes e do caule, respectivamente. Os estudos de caracterização através da espectroscopia UV-vis possibilitaram conhecer os espectros de absorção para cada corante e a técnica de difração de raios X permitiu a caracterização estrutural da camada semicondutora nanoestruturada. As células solares foram caracterizadas quanto a seus parâmetros de eficiência (Voc, Jsc, FF e ? (%)), obtidas das curvas de corrente vs potencial. Tais parâmetros se mostraram modestos, apresentando valores de Voc sobre os 0,334 volts e Jsc de 0,452 mA/cm2 para célula fotossensibilizada com o corante extraído da Genipa americana. Dessa forma foi possível verificar o potencial fotoeletroquímico dos corantes extraídos de plantas da Floresta Amazônica.

ABSTRACT In this work, plant dyes extracted from Amazon Forest are applied as a sensitizer in Dye-Sensitized Solar Cells. The selected plants were Euterpe oleracea, Arrabidaea chica, Bixa orellana, Genipa Americana, and Myrcia sylvatica, and the dyes were collected from fruits, leaves, seeds, pulp and seeds and stalk scrapings respectively. Characterization studies by the UV-vis spectroscopy made it possible to know the absorption spectra of each plant dye, and the X-ray diffraction technique allows the structural characterization of the nanostructured semiconductor layer. The solar cells were characterized according to their efficiency parameters (Voc, Jsc, FF and ? (%)), obtained from the current vs voltage curves. Such parameters proved to be modest, presenting Voc and Jsc values over 0.334 volts and 0.452 mA/cm2 for a photosensitized cell with the dyes extracted from Genipa americana. In this way, it was possible to verify the photoelectrochemical potential of the dyes extracted from plants of the Amazon Forest.

Abstract This study evaluates the shear bond strength (SBS) of various resin cements to different ceramics. Composite resin cylinders of Z100 were fabricated and cemented to disks of feldspathic ceramic (Creation), leucite-reinforced feldspathic ceramic (Empress I), and densely sintered aluminum oxide ceramic (Procera AllCeram) using five resin cements: Panavia F (PAN), RelyX ARC (ARC), RelyX Unicem (RXU), RelyX Veneer, and Variolink II. SBS was measured after three days of water storage (baseline) and after artificial aging (180 days of water storage along with 12,000 thermal cycles). Failure mode of fractured specimens also was evaluated. Data were analyzed with Kruskal-Wallis and Mann-Whitney tests (α=0.05). RXU showed 1) the lowest baseline median SBS to feldspathic ceramic, which was not statistically different from PAN; 2) the lowest median baseline SBS to leucite-reinforced feldspathic and densely sintered aluminum-oxide ceramics. All cements performed similarly after aging, except for ARC (median 0.0 MPa) and PAN (median 16.2 MPa) in the densely sintered aluminum-oxide ceramic group. Resin cements perform differently when bonded to different ceramic substrates. While all test resin cements worked similarly in the long-term to feldspathic and leucite-reinforced feldspathic ceramics, only the MDP-containing resin cement provided durable bonds to densely sintered aluminum-oxide ceramic.

Resumen Introducción. Aedes aegypti es el vector de los virus del dengue, la fiebre amarilla, el Zika y el chikungunya, y Culex quinquefasciatus, de los virus de la encefalitis de Saint Louis y de la del Oeste del Nilo. Objetivo. Evaluar la capacidad infecciosa de Heterorhabditis bacteriophora N4 en larvas de C. quinquefasciatus y A. aegypti en el laboratorio. Materiales y métodos. Treinta larvas de segundo estadio de cada especie de mosquito se expusieron a diferentes dosis (0:1, 1:1, 5:1, 15:1, 100:1, 500:1, 750:1 y 1.500:1) de ejemplares juveniles infectivos del nematodo. Se hicieron cuatro réplicas por dosis. Resultados. El parasitismo varió entre 2,5 y 80 % en C. quinquefasciatus y entre 4,2 y 92,5 % en A. aegypti, con diferencias significativas entre las dosis (p<0,0001). Las DL50 fueron de 160,8 ejemplares juveniles infectivos por larva de C. quinquefasciatus y 113,6 por larva de A. aegypti; 4 a 6 % de los ejemplares juveniles infectivos llegaron a la fase adulta en C. quinquefasciatus, y 12 a 61 % en A. aegypti. Nuevos ejemplares juveniles infectivos aparecieron a partir de la dosis de 100:1. En A. aegypti aparecieron solo con dosis superiores a esta y, en C. quinquefasciatus, con una dosis de 1500:1. Se observó melanización de los ejemplares juveniles infectivos en las dos especies de mosquitos. Conclusión. Se estableció la sensibilidad de estas especies de mosquitos al parasitismo producido por un aislamiento autóctono de H. bacteriophora en condiciones de laboratorio y su potencial efecto como agente de control biológico.

Abstract Introduction: Aedes aegypti is the vector of dengue, yellow fever, Zika and Chikungunya viruses, and Culex quinquefasciatus is the vector of St. Louis and West Nile encephalitis viruses. Objective: To evaluate infectivity of Heterorhabditis bacteriophora N4 in C. quinquefasciatus and A. aegypti larvae under laboratory conditions. Materials and methods: Thirty second-instar larvae of the two mosquito species were exposed each to different doses (0:1, 1:1, 5:1, 15:1, 100:1, 500:1, 750:1 and 1,500:1) of nematode infective juveniles. Four replications per dose were performed. Results: Parasitism varied between 2.5 and 80 % in C. quinquefasciatus, and between 4.2 and 92.5 % in A. aegypti, with significant differences between doses (p<0.0001). DL50 were: 160.8 infective juveniles per larva for C. quinquefasciatus and 113.6 infective juveniles per larva for A. aegypti. In C. quinquefasciatus, 4 to 6 % of the infective juveniles developed to adults and in A. aegypti, 12- 61 %. In A. aegypti the emergence of new infective juveniles occurred with 100:1, 500:1, 750:1 and 1,500:1 infective juveniles per larva, and in C. quinquefasciatus, with 1,500:1 infective juveniles per larva. Melanization of infective juveniles was observed in both mosquito species. Conclusion: The susceptibility of these mosquito species to parasitism of an indigenous isolate of H. bacteriophora in the laboratory was demonstrated. Heterorhabditis bacteriophora N4 could be an efficient biological control agent.

We report the presence of the competent vector for Leishmaniaspp, Migonemyia migonei, and theEvandromyia cortelezzii-sallesi complex south of its known distribution in the central temperate region of Argentina, in the province of Córdoba. The persistence of this phlebotomine in the northern border of the province, its association with a case of cutaneous leishmaniasis, and the new record in the outskirts of the city of Córdoba, the second most populated in the country, strengthens the need for regular vector surveillance and a case detection-sensitive health system in vulnerable regions, even in temperate climates.

Objective: To compare gestational age assessed by Capurro test versus the gold standard, the first trimester ultrasound (10+0 to 14+2 weeks) in women with reliable last menstrual period. Design: Retrospective and correlation study. Setting: Instituto Nacional Materno Perinatal, Lima, Perú. Participants: Fetuses and the same once born. Methods: Ultrasound study was performed to 167 pregnant women at 10+0 to 14+2 weeks of gestation during the period 2005 through 2009; gestational age at birth of fetuses calculated by ultrasound was compared with gestational age by Capurro test. Main outcome measures: Comparison of medians of Wilcoxon test, interclass correlation coefficient, Lin’s coefficient concordance correlation, Bland & Altman’s graphic limits of agreement. Results: Mean gestational age assessed by Capurro test was 39 weeks versus 38 weeks determined by the crown rump length (Wilcoxon test z=-3.88, P<0.01). The intraclass correlation coefficient was 0.67 (95%CI: 0.58-0.75), the Lin’s coefficient concordance correlation was 0.65 (95%CI: 0.55-0.72), and the Bland & Altman limits of agreement was -0.41 (95%LOA -2.9-2.1). Conclusions: Gestational age assessment by Capurro test tended to overestimate age. The relation between these two methods was not perfect as measurements were different.

Objetivos: Comparar la edad gestacional determinada por la evaluación pediátrica al nacimiento (test de Capurro) y la prueba de oro, la ecografía del primer trimestre (10 +0 a 14+2 semanas) en gestantes con FUR confiable. Diseño: Estudio retrospectivo de correlación. Institución: Instituto Nacional Materno Perinatal, Lima, Perú. Participantes: Fetos y los mismos recién nacidos. Métodos: A 167 gestantes se les realizó ecografía a las 10+0 a 14+2 semanas en el período de 2005 al 2009; la edad gestacional al nacer de estos fetos se comparó con la edad gestacional al nacer por la prueba de Capurro. Principales medidas de resultados: Comparación de medianas con la prueba de Wilcoxon, coeficiente de correlación interclase, coeficiente de concordancia de correlación, repetibilidad o acuerdo según gráfico de Bland & Altman. Resultados: La mediana de edad gestacional calculada por Capurro fue 39 semanas versus la longitud corono nalga de 38 semanas ((Wilcoxon test z=-3,88, P<0,01). El coeficiente de correlación intraclase fue 0,67 (IC95%: 0,58 a 0,75), el coeficiente de correlación de concordancia de Lin (0,65 (IC95%: 0,55 a 0,72) y los límites de acuerdo de Bland & Altman -0,41 (LOA95% -2,9 a 2,1). Conclusiones: La edad gestacional determinada por el examen de Capurro tiende a sobreestimar la edad gestacional A pesar de que hay una relación directa entre estos dos métodos, la correlación no es perfecta y ambos métodos no son repetibles, como lo demuestran los diversos coeficientes calculados.

The discovery of cell free fetal DNA (cff-DNA) in maternal circulation has profoundly changed the clinical practice of Obstetrics and knocked down an old paradigm in prenatal diagnosis. Over the past five years screening for chromosomal abnormalities has moved from one with a high false positive rate into another more effective and at lower false positive rate. Undoubtedly, this test is the best effective screening tool in Obstetrics.

El descubrimiento del ADN libre fetal circulando en sangre materna ha revolucionado la práctica de la Obstetricia y cambiado el paradigma del diagnóstico prenatal. En los últimos 5 años hemos avanzado del tamizaje de aneuplodías con muchos falsos positivos a uno mucho más efectivo y con cifras de falsos positivos muy bajas. Este es, sin duda, el mejor método de tamizaje actual que se tiene en Obstetricia.

Studies were conducted to determine the selection of humans, chickens and rabbits by Culicidae in three different environments in the province of Chaco, Argentina. Mosquitoes were collected fortnightly using cylindrical metal traps containing animal bait (chickens and rabbits). The mosquitoes were collected between June 2001-May 2002. During the same period and with the same frequency, mosquitoes biting the human operators of the traps were collected during the first 15 min of exposure within different time intervals: from 09:00 am-11:00 am, 01:00 pm-03:00 pm, 05:00 pm-07:00 pm and 09:00 pm-10:00 pm. A total of 19,430 mosquitoes of 49 species belonging to 10 genera were collected. Culex species mainly selected chicken bait and Wyeomyia species selected rabbit bait. Ochlerotatus and Psorophora species were more abundant in rabbit-baited traps. Anopheles triannulatus, Coquillettidia nigricans, Ochlerotatus scapularis, Mansonia titillans and Psorophora albigenu showed a strong attraction for human bait. The Anopheles, Coquillettidia, Culex and Mansonia species were more active between 05:00 pm-09:00 pm, while Ochlerotatus, Psorophora, Haemagogus and Wyeomyia were most active from 09:00 am-07:00 pm. This study provides additional information about the biology and ecology of arbovirus vectors in Chaco.

O objetivo deste estudo foi avaliar a absorção de água e a solubilidade de diferentes sistemas adesivos: Adper Easy Bond, Adper Single Bond Plus, Bond Force, Clearfil SE Bond (apenas a resina adesiva) e Xeno IV. Foram fabricados 10 discos de cada material em moldes de Teflon e foi permitida a evaporação de todos os componentes voláteis. Os discos foram pesados diariamente em balança analítica até atingir massa seca constante (m1). Após esta mensuração, os discos foram imersos em água por 12 meses e seu peso úmido foi anotado (m2). Os discos foram novamente pesados diariamente até obter-se massa constante (m3). As percentagens de absorção de água e solubilidade foram calculadas utilizando os valores de massa registrados. A comparação das médias de absorção de água e solubilidade entre os diversos adesivos foi feita com o teste Kruskal-Wallis. As diferenças de absorção de água e solubilidade entre os pares de adesivos foram determinadas pelo testes Mann-Whitney com correção de Bonferroni. O nível de significância adotado foi de 0,05. Absorção de água e solubilidade apresentaram diferenças estatisticamente significantes entre os grupos (p<0,05). As comparações pareadas dos adesivos não mostraram diferenças significantes (p>0,05) entre Adper Single Bond Plus e Bond Force nem entre Clearfil SE Bond e Xeno IV para absorção de água e solubilidade. Xeno IV apresentou diferença significante do Adper Easy Bond quanto à absorção de água (p>0,05). A absorção de água e solubilidade dos adesivos “all-in-one” aumentaram com o tempo e as taxas de aumento mostraram-se dependentes da composição do material. Os resultados sugerem que outros monômeros além do HEMA contribuem para a absorção de água e solubilidade dos diversos sistemas de adesivos.

The aim of this study was to evaluate the water sorption and solubility of different adhesives. Adper Easy Bond, Adper Single Bond Plus, Bond Force, Clearfil SE Bond (bonding resin only), and Xeno IV were the materials evaluated. Ten disks of each adhesive were made in Teflon molds and evaporation of any volatile components was allowed. The disks were weighed daily in an analytical balance until a constant mass was obtained (m1). Disks were then immersed in water for 12 months when their wet weight was recorded (m2). The disks were again weighed daily until a constant mass was obtained and the final weight recorded (m3). Water sorption and solubility (percentages) were calculated using the recorded mass values. Kruskal-Wallis tests were used to compare the average water sorption and solubility among the different adhesives. Mann-Whitney tests with a Bonferroni correction were used to determine the pairwise differences between adhesives in water sorption and solubility. The level of significance was set at 0.05. Water sorption and solubility were significantly different among the groups (p<0.05). Pairwise comparisons showed no significant differences (p>0.05) between Adper Single Bond Plus and Bond Force, or between Clearfil SE Bond and Xeno IV in either water sorption or solubility. Xeno IV did not differ from Adper Easy Bond in water sorption (p>0.05). Water sorption and solubility of all-in-one adhesives increased with time, and the rates of increase were composition-dependent. The results suggest that monomers other than HEMA contribute to water sorption and solubility of adhesive systems from different categories.

OBJETIVO: Analisar os resultados obstétricos no segundo parto de mulheres que já haviam realizado uma cesariana. MÉTODOS: Estudo de coorte retrospectivo em um hospital materno. Foram incluídas mulheres grávidas que deram à luz (vaginal ou cesárea) de 2001 a 2009. Os principais critérios de inclusão foram: mulheres com 24 a 41 semanas de gestação e com um parto prévio. Duas coortes foram selecionados, sendo uma incluindo mulheres com uma cesariana anterior (n=7.215) e outra com um parto vaginal (n=23.720). Ambos os grupos foram comparados, e uma regressão logística foi realizada para ajustar devido às variáveis de confusão. Os resultados obstétricos incluídos foram ruptura uterina, placenta prévia, complicações relacionadas com uma placentação inadequada, tais como descolamento prematuro da placenta, pré-eclâmpsia e parto prematuro espontâneo. RESULTADOS: Mulheres com uma cesariana anterior foram mais propensas a ter resultados adversos, tais como ruptura uterina (OR=12,4, IC95% 6,8-22,3), descolamento prematuro da placenta (OR=1,4, IC95% 1,1-2,1), pré-eclâmpsia (OR=1,4, IC95% 1,2-1,6) e parto prematuro espontâneo (OR=1,4, IC95% 1,1-1,7). CONCLUSÕES: Pessoas com uma cesárea anterior têm resultados obstétricos adversos na gravidez subsequente, incluindo ruptura de útero, distúrbios relacionados com uma placentação inadequada, tais como pré-eclâmpsia, parto prematuro espontâneo e descolamento prematuro da placenta.

PURPOSE: To examine obstetric outcomes in the second birth of women who had undergone a previous cesarean delivery. METHODS: This was a large hospital-based retrospective cohort study. We included pregnant women who had a previous delivery (vaginal or cesarean) attending their second birth from 2001 to 2009. Main inclusion criteria were singleton pregnancies and delivery between a gestation of 24 and 41 weeks. Two cohorts were selected, being women with a previous cesarean delivery (n=7,215) and those with a vaginal one (n=23,720). Both groups were compared and logistic regression was performed to adjust for confounding variables. The obstetric outcomes included uterine rupture, placenta previa, and placental-related complications such as placental abruption, preeclampsia, and spontaneous preterm delivery. RESULTS: Women with previous cesarean delivery were more likely to have adverse outcomes such as uterine rupture (OR=12.4, 95%CI 6.8-22.3), placental abruption (OR=1.4, 95%CI 1.1-2.1), preeclampsia (OR=1.4, 95%CI 1.2-1.6), and spontaneous preterm delivery (OR=1.4, 95%CI 1.1-1.7). CONCLUSIONS: Individuals with previous cesarean section have adverse obstetric outcomes in the subsequent pregnancy, including uterine rupture, and placental-related disorders such as preeclampsia, spontaneous preterm delivery, and placental abruption.

Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species.

Rio Negro virus (RNV) (Venezuelan equine encephalitis subtype VI) circulates only in Argentina; in northern provinces, isolates have been obtained from mosquitoes and rodents since 1980 and have been associated with acute febrile illness in humans. However, no studies of RNV have been performed in the central area of the country. We carried out molecular and serological detection of RNV in Córdoba, a province of the central part of the country, in mosquitoes and humans, respectively. One mosquito pool tested positive for alphavirus RNA by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR). Subsequent sequencing determined that this alphavirus grouped with RNV. Serological studies detected antibodies to RNV in one human serum sample, which was obtained during the same period that RNV was detected using the aforementioned molecular methods. This is the first report of RNV circulation in the central area of Argentina, indicating an expansion of its original distribution. These results highlight the importance of strengthening surveillance procedures in endemic areas, as well as in new regions where RNV may emerge.

O objetivo do estudo foi determinar os horários de máxima atividade de oviposição de Aedes aegypti na cidade de Orán (noroeste argentino). Foram realizadas coletas quinzenais, entre novembro de 2006 e fevereiro de 2007 (primavera-verão). A cidade foi dividida em três zonas (norte, centro, sul); foram escolhidos aleatoriamente dez domicílios em cada zona e duas ovitrampas foram instaladas no exterior de cada domicílio. As ovitrampas eram trocadas a cada quatro horas, cobrindo desde a manhã (8h) até a noite (20h). A maior quantidade de ovos (81%) foi registrada entre as 16h e 20h. Esses dados aportam conhecimento do vetor e, portanto, a seu controle, como, por exemplo, realizar a fumegação na faixa horária de maior atividade de oviposição.

The study aimed to determinate the maximum daily peak of Aedes aegypti oviposition in the city of Oran, northwestern Argentina. Biweekly samplings were taken between November 2006 and February 2007 (spring-summer). The city was divided into three areas (north, center, and south) and households were randomly selected. Two ovitraps were placed outdoors in the selected houses. Ovitraps were replaced every four hours, from morning (8 a.m.) to late afternoon (8 p.m.). The largest number of eggs was recorded between 4 p.m. and 8 p.m. (81%). These findings enhance our understanding of the vector and thus its control such as spraying during the hours of peak oviposition activity.

El objetivo del estudio fue determinar los horarios de máxima actividad de oviposición de Aedes aegypti en la ciudad de Orán (noroeste argentino). Se realizaron muestreos quincenales entre noviembre de 2006 y febrero de 2007 (primavera-verano). La ciudad fue dividida en tres zonas (norte, centro, sur), donde fueron seleccionadas al azar las viviendas para colocar dos ovitrampas en el exterior de las mismas. Las ovitrampas fueron reemplazadas cada cuatro horas, desde la mañana (8h) hasta el atardecer (20h). Se registró la mayor cantidad de huevos entre las 16 y 20 h (81%). Estos datos aportan al conocimiento del vector y, por lo tanto, de la posibilidad de control, pudiendo realizarse las fumigaciones en la franja horaria con mayor actividad de oviposición.

O objetivo do estudo foi determinar os horários de máxima atividade de oviposição de Aedes aegypti na cidade de Orán (noroeste argentino). Foram realizadas coletas quinzenais, entre novembro de 2006 e fevereiro de 2007 (primavera-verão). A cidade foi dividida em três zonas (norte, centro, sul); foram escolhidos aleatoriamente dez domicílios em cada zona e duas ovitrampas foram instaladas no exterior de cada domicílio. As ovitrampas eram trocadas a cada quatro horas, cobrindo desde a manhã (8h) até a noite (20h). A maior quantidade de ovos (81%) foi registrada entre as 16h e 20h. Esses dados aportam conhecimento do vetor e, portanto, a seu controle, como, por exemplo, realizar a fumegação na faixa horária de maior atividade de oviposição.

The study aimed to determinate the maximum daily peak of Aedes aegypti oviposition in the city of Oran, northwestern Argentina. Biweekly samplings were taken between November 2006 and February 2007 (spring-summer). The city was divided into three areas (north, center, and south) and households were randomly selected. Two ovitraps were placed outdoors in the selected houses. Ovitraps were replaced every four hours, from morning (8 a.m.) to late afternoon (8 p.m.). The largest number of eggs was recorded between 4 p.m. and 8 p.m. (81%). These findings enhance our understanding of the vector and thus its control such as spraying during the hours of peak oviposition activity.

El objetivo del estudio fue determinar los horarios de máxima actividad de oviposición de Aedes aegypti en la ciudad de Orán (noroeste argentino). Se realizaron muestreos quincenales entre noviembre de 2006 y febrero de 2007 (primavera-verano). La ciudad fue dividida en tres zonas (norte, centro, sur), donde fueron seleccionadas al azar las viviendas para colocar dos ovitrampas en el exterior de las mismas. Las ovitrampas fueron reemplazadas cada cuatro horas, desde la mañana (8h) hasta el atardecer (20h). Se registró la mayor cantidad de huevos entre las 16 y 20 h (81%). Estos datos aportan al conocimiento del vector y, por lo tanto, de la posibilidad de control, pudiendo realizarse las fumigaciones en la franja horaria con mayor actividad de oviposición.